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The Cleavage Control Protein is used to monitor performance of either thrombin or enterokinase cleavage conditions. The 48 kDa protein is cleaved into 2 fragments or cleaved by , which are easily visualized by SDS-PAGE. Thrombin cleavage results in fragments of 35 kDa and 13 kDa, and Enterokinase cleavage results in fragments of 32 kDa and 16 kDA.

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